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1. How can you protect a pipette from inner contamination?
Use filter tips and a cautious working approachSpray disinfectant into the pipetteYou cannot protect a pipette from inner contamination

2. Which general recommendations for pipetting are important?

Vertical aspiration, low immersion depth, pre-wetting and dispensing to vessel wallAlways holding the pipette at an angle and aspirating as fast as possibleThere are no general recommendations for pipetting

3. Which liquid handling instrument can be used for titration with a negligible chance of human error?

PipetteE-buretteBottle Top Dispensers

4. Which of these is a good practice for pipette maintenance?

Store vertically in a pipette holderDo not set on its side with liquidUse well-fitting tipsAll of the above

5. Which effect can liquids with high vapour pressure, e.g. acetone have on air cushion pipetting?

NoneThe vapour can expand the air cushion and lead to drippingThe vapour can expand the tip and it will fall off

6. When using a micropipette, if you push to the second stop to fill it, you will get:

The correct amount of solutionToo much solutionToo little solutionEither too much or too little solution

7. You should NEVER rotate the volume adjuster on a micropipette beyond the upper and lower limit

TrueFalse

8. Which ISO Standard do we follow at Microlit for stringent validation and calibration procedures for pipettes?

ISO/IEC 8655ISO 13485:2003ISO 9000

9. What is CV (coefficient of variation) in liquid handling terms?

Systematic error - how far away from specified volumeRandom error; closeness of respective aliquot volumes; reproducibility