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What is reverse pipetting technique?

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Reverse pipetting is a technique used to dispense a precise and small quantity of liquid by means of air displacement. This technique reduces the risk of splashing, foaming or bubble formation in the liquid.

B scaled

  1. To Aspirate the sample in the tip, press the plunger to the second stop and immerse the micropipette tip vertically in the liquid.
  2. Slowly release the plunger while the tip is immersed. The sample will be aspirated into the micropipette tip.
  3. To dispense the sample, place the tip on the inner wall of the receiving vessel at a steep angle.
  4. Slowly press the plunger to the first stop.
  5. Wipe the tip on the inner wall while taking the tip out of the receiving vessel.