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What are the different techniques of pipetting?

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There are mainly two techniques of pipetting:

  1. Forward pipetting technique: Forward pipetting is a technique for dispensing a precise amount of liquid by means of an air displacement pipette. The technique is primarily recommended for aqueous solutions, such as buffers, or dilute acids or alkalis.Pipette techniques
  2. Reverse pipetting technique: Reverse pipetting is a technique for dispensing a precise amount of liquid by means of an air displacement pipette. This technique is mainly used for solutions with high viscosity or a tendency to foam as it minimizes the risk of splashing, foam, or bubble formation. Reverse piping is more accurate in dispensing small amounts of liquids containing proteins and biological solutions.